Regular™ Rabbit Antibody Service
The quantity and the type of antibody needed, as well as the nature and the amount of available immunogens are common determining factors for selecting appropriate animal hosts for immunization. For polyclonal antibodies directed against peptide immunogens, rabbits are usually the suitable and most commonly chosen immunization host. However, individual rabbits can always exhibit both quantitative and qualitative differences in their antibody response to the same peptide immunogen. Therefore, it is advisable that multiple rabbits be used for each peptide immunogen.
($15 per amino acid over 15 residues)
Investigators are also welcome to provide their own ready-to-use immunogen for antibody production. For Regular™ rabbit antibody production in 2 rabbits using an investigator provided ready-to-use immunogen, we ask that we are provided with 5 mg of the immunogen at a concentration of no less than 0.5 mg/ml preferably in 1X PBS, though any buffer is acceptable provided it does not contain high concentrations of denaturing agents (e.g. >4M Urea). Please contact us if the quantity of your immunogen is limiting. Also, we strongly encourage investigators to contact us prior to submitting their immunogen to ensure antibody production in rabbits is suitable to raise a potentially useful antibody.
The price for Regular™ Rabbit Antibody Service using an investigator provided ready-to-use immunogen in 2 rabbits is $600 per project.
Our animal facility is NIH/OLAW/PHS assured, USDA certified, and IACUC regulated.
For projects that require animal work to be conducted at an AAALAC accredited animal facility, please contact us.
Due to its synthetic nature, the chemically synthesized peptide, when used as an immunogen, may not necessarily achieve the same effect as that of the native protein. The elicited antibody may not necessarily perform up to the investigator’s expectations due to a variety of factors that may exist in the investigators assay system (e.g. unknown post-translational modifications, low endogenous protein levels, protein processing events, reagents used, etc.). YenZym ensures only the quality of the peptide synthesized (by mass spectrometry) and guarantees specificity of the antibody response to the immunizing peptide (by ELISA). However, YenZym will try its best to help remedy the situation should antibody difficulty be encountered.
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