CUSTOM ANTIBODY SERVICES

P-site™ Antibody Service

Post-translational modifications (PTMs) play important roles in regulating the biological activity of a protein. Phosphorylation is one of the most common PTMs carried out by the protein kinases. A properly designed P-site™-specific antibody allows one to monitor the kinase activation event without the use of radioisotope 32p.  

Our team of scientists has derived a wealth of experience over the years by making hundreds of P-site™-specific antibodies (including methylations (me1, me2, and me3), acetylations, hydroxylations, neo-epitopes, etc.). The design of the P-site™ epitope critically determines the outcome (yield, specificity and affinity) of the final antibody. Based on your individual project needs, our team will tailor the epitope design accordingly.

For a singly-phosphorylated peptide of 15 residues in length:

Yen-de-Zyn of P-site™ epitope
$195FREE
Synthesis of phosphorylated peptide:
  • HPLC high purity peptide will be used for immunization purposes.
  • General grade peptide for ELISA and Affinity Purification purposes.
  • Remaining amount of peptide will be provided to the investigator.
  • $650

    ($25 per extra residue when exceeding 15 residues, should epitope design call for it.)

    Synthesis of non-phosphorylated peptide counterpart:
  • General grade peptide will be used for ELISA and affinity absorption purposes.
  • Remaining amount of peptide will be provided to the investigator.
  • $200
    Carrier Protein Conjugations of:
  • HPLC-highly purified phosphorylated peptide to carrier protein (KLH or THY) for immunization,
  • HPLC-highly purified phosphorylated peptide to GIgG for ELISA, and
  • General grade non-phosphorylated peptide counterpart to GIgG for ELISA.
  • $250
    P-site™ Regular™ Antibody production (2 Rabbits):
  • Regular™ Antibody Production regimen
  • ~5 ml pre-immune serum per rabbit
  • ~5 ml test bleed serum per rabbit
  • Pre-immune vs. Test Bleed ELISA against phosphorylated and non-phosphorylated peptides
  • ~50 ml final antiserum per rabbit
  • $800
    Step 1. Phosphorylated peptide-specific Affinity Purification of Antibody:
  • Preparation of phosphorylated-peptide conjugated affinity matrix
  • Separate purification of antiserum from both rabbits
  • Affinity matrix will be provided to the investigator upon completion of project
  • $795
    Step 2. Affinity Absorption of Step 1-purified Antibody to separate P-site™-specific antibody from the cross-reactive antibody populations
  • Preparation of non-phosphorylated peptide conjugated affinity matrix
  • Separate absorption of both Step 1 purified antibodies
  • ELISA of pre- and post-purified serum to ensure depletion of specific antibody and ELISA of purified and absorbed antibody preparations against phosphorylated and non-phosphorylated peptides to ensure P-site™ specificity
  • SDS-PAGE of final antibody preparations
  • Affinity matrix will be provided to the investigator upon completion of project
  • $795
    TOTAL
    $3,490 $2,695*

    *Pricing will be dependent upon type and/or number of modifications and epitope length.

    Terms and Conditions